Analytical Sciences

Glutathione Peroxidase-Like Catalytic Activities of Ion-Exchange Resins Modified with Metalloporphyrins

Akimasa IWADO*, Masaki MIFUNE**, Rumiko HARADA (nee NOGUCHI)**, Takashi MUKUNO*, Noriko MOTOHASHI*** and Yutaka SAITO** *

*The Graduate School of Natural Science and Technology, Okayama University, Tsushima-Naka, Okayama 700, Japan
**Faculty of Pharmaceutical Sciences, Okayama University, Tsushima-Naka, Okayama 700, Japan
***Kobe Pharmaceutical University, Motoyamakita-Machi, Higashinada, Kobe 658, Japan

Some of the ion-exchange resins modified with metalloporphyrins have been found to exhibit glutathione-peroxidase (GSHPx)-like catalytic activities for hydrogen peroxide and cumene hydroperoxide in the oxidative reaction of glutathione to disulfide. In particular, the anion-exchange resin modified with Mn(III)-tetrakis(sulfophenyl)porphine (Mn-TSPPr) exhibited stronger activity than GSHPx, and hence Mn-TSPPr was expected to be a good mimesis in place of GSHPx or immobilized GSHPx for the determination of peroxide. (Keywords: Glutathione-peroxidase-like activity, ion-exchange resin modified with metalloporphyrin, hydrogen perox-ide, cumene hydroperoxide)

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