Analytical Sciences

Abstract − Analytical Sciences, 14(2), 355 (1998).

Retention Behavior of p-Alkylphenols in Reversed-Phase Liquid Chromatography Using Ethanol/Water Mixtures
Kanji MIYABE * , Noboru NOMURA, Fumie MORISHITA, Sugako KURATA, Hiroko ASAMURA and Yukiko IMADA
Chemistry Section, Faculty of Education, Toyama University, Gofuku, Toyama 930, Japan
The retention behavior of p-alkylphenols was studied in reversed-phase liquid chromatography using octadecylsilyl-silica gel and ethanol/water mobile phases. The results were compared with those in methanol/water systems. Linear correla-tions were observed between the logarithmic retention factor (ln k) and the carbon number of side chains in the solutes, irrespective of the type and volumetric fraction ( f) of the organic modifiers in the mobile phases. The straight lines mea-sured at different fconverged at almost one point. By analyzing the slope of the linear correlations, a functional contri-bution of a methylene group to k and a reduction of the hydrophobic surface area of the solutes and octadecylsilyl ligands due to the retention were estimated. It was also attempted to analyze the characteristics of the retention in reversed-phase liquid chromatography on the basis of solvophobic theory. (Keywords: Reversed-phase liquid chromatography, retention factor, solvophobic theory, retention contribution, p-aklylphenol, alcohol/water eluent)