`BUNSEKI KAGAKU' 2002. 6

B U N S E K I K A G A K U

Vol. 51 No. 6

June, 2002

Accounts

Development of Chiral Derivatization Reagents Having Benzofurazan (2,1,3-Benzoxadiazole) Fluorophore for HPLC Analysis and Their Application to the Sensitive Detection of Biologically Important Compounds

Toshimasa Toyo'oka 339

Recognition of Biological Substrates Based on Supramolecular Interation and Its Application to Chirality Sensing with Circular Dichroism Spectroscopy

Hiroshi Tsukube, Tomoko Nishimura and Satoshi Shinoda 359

Enzyme Activity Analysis of Soluble Proteins Using Non-Denaturing Two-Dimensional Electrophoresis

Youji Shimazaki, Yoko Hiraka, Hiromi Ohnishi and Takashi Manabe 367

Detection of Environmental Mutagens Using Transgenic Animals

Yasunobu Aoki, Hiromi Sato and Kimiko Amanuma 373

Research Papers

Rapid Simultaneous Detection of Verotoxin 1 and 2 Using Time-Resolved Fluoroimmunoassay

Kazuyuki Watanabe, Hidetoshi Arakawa and Masako Maeda 381

Screening Method of Endocrine Disrupting Chemicals Using a Surface Plasmon Resonance Sensor

Kazunobu Asano, Atsushi Ono, Setsuko Hashimoto, Tohru Inoue and Jun Kanno 389

Determination of Paraben Found on Skin Surface of Human Finger

Hideki Shibata and Takao Tsuda 397

Electrochemical Responses of Catecholamines at a Cyclodextrin Monolayer-Modified Electrode

Iwao Suzuki, Yuya Egawa and Jun-ichi Anzai 403

Electrode Reaction of Catecholamines on a Calix[6]arene Monolayer Electrode and Its Application to a Doapmine Sensor

Koji Nakano, Sachiko Tanaka, Makoto Takagi and Seiji Shinkai 409

Total Free Catecholamines Assay by Using a Minicolumn as an Off-Line Reactor

Hiroko Kawamoto and Osamu Nozaki 415

Determination of Glucose and Uric Acid by FIA with Electrochemiluminescence with High-Sensitivity

Touru Shinohara, Akira Kotani and Fumiyo Kusu 423

Determination of Lactic Acid, Uric Acid, Xanthine and Tyrosine in Human Sweat by HPLC, and the Concentration Variation of Lactic Acid in it after the Intake of Wine

Sadaharu Sugase and Takao Tsuda 429

Molecular Species Analysis of 1,3-Diacylglycerols in Edible Oils by HPLC/ESI-MS

Hiroyuki Takano and Yutaka Itabashi 437

Development of an Analytical Method for Intermediates in Bile Acid Biosynthesis by GC/MS

Tsuyoshi Murai, Kana Yamaguchi, Teruki Yoshimura, Shu-Ping Hui and Takao Kurosawa 443

Amperometric Measurement of the Microbial Substrate-Oxidizing Activity of Microbial Cells Exposed to Bactericidal Stress

Tetsuya Kondo, Kumiko Kawai, Kyoden Yasumoto and Tokuji Ikeda 449

Immobilization of Horseradish Peroxidase on Nanometer-Scale Domains of Phase-Separated Binary Self-Assembled Monolayers Formed by Coadsorption on Au(111)

Daisuke Hobara, Yusuke Uno and Takashi Kakiuchi 455

Technical Papers

Simultaneous Analysis of Drug Efficacy and Early Absorption, Distribution, Metabolism and Excretion Characterization Using Surface Plasmon Resonance Sensor

Junichi Inagawa and Kaori Morimoto 461

Notes

Analytical Evaluation of Catecholamine Biosensors Based on Tyrosinase from Mushroom and Mushroom Tissue

Toshio Yao and Chong-Ok Ho 469

Technical Letters

An Attempt of pH Mapping on Biological Specimens by pH-Imaging Microscope Using a Semiconductor Silicon Sensor

Yukiko Nakano, Satoshi Nomura, Tadafumi Kawamoto, Junji Tagami, Atsushi Horiba and Yoshiro Takano 473

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